HiCC-S fully automatic colony counting and inhibition zone measuring instrument
1、 Main performance parameter indicators:
1. Imaging device
1) Equipped with a solid-state drive computer and an autofocus large depth of field 8-megapixel (3264x2448 pixels) color shooting light box capable of macro shooting, with a maximum resolution of 0.02mm, it can recognize bacteria as small as 0.05mm. Paired with Epson V330 Ultra Micro Stereoscopic Color Scanning Imager (19mm large depth of field, micro lens 12 line matrix CCD, adjustable brightness, automatic focusing), it can achieve floating dark field imaging with an optical resolution of 40 million pixels and a maximum color depth of 48 bits
2) Fully enclosed dark box to eliminate environmental stray light interference
3) Shooting lightbox with flexible shadowless upper light source（Adjustable 20 color LED surround structured light+8-level brightness adjustment）To create a clear suspended dark field colony imaging effect. Uniform and shadowless illumination from a lower light source to highlight the morphology of bacterial colonies and achieve intelligent recognition and counting in a foolproof manner
4) Shooting lightboxesUpper light source, lower light source, upper and lower dual light sources, UV light source (used for cavity disinfection and UV mutagenesis), can be freely combined and independently switchedTo obtain the best colony imaging
5) Suitable for culture dish diameter: 50-150mm flat dish (pouring, membrane filtration, 3M paper sheet)
2. Colony count analysis
1)★Automatic recognition and statistics: SmartdownTM colony intelligent recognition technology has the characteristic of automatic enhancement of colony targets, which can automatically recognize large-scale clustered and chain shaped adhered long rod-shaped bacteria (microscopic morphology of large adhered Escherichia coli, bacterial spores) and various types of colonies on petri dishes (including Staphylococcus aureus count (GB4789.10-2016), Escherichia coli group determination (GB4789.3-2016), etc.). Automatically generate batch processing wizard to achieve one click automatic counting
2) The automatic counting accuracy is ≥ 96.5%, with a maximum monitoring correction of 3.5%, achieving 100% accuracy. Analysis and statistics speed: 150-1000 colonies/s, total time for plate imaging and counting ≤ 4 seconds
3)★Colony adhesion segmentation: Automatically segment long, circular, and other colony targets that are adhered to each other, and users can choose to segment or not segment them. It is also possible to manually segment and merge colony target images
4)★Color and Shape Recognition: With fast level set extraction technology, it can classify and count the number of bacterial colonies of specific colors and shapes according to 20 categories, and can be corrected by category conversion. 6 target areas can be selected simultaneously for automatic analysis, and the analysis target areas can be freely increased, decreased, or edited
5) Colony morphology analysis: Automatically obtain colony area, equivalent diameter, major axis, minor axis, aspect ratio, roundness, perimeter, shape factor, etc. You can filter and query based on morphological indicators.
6) Mouse click correction, seamless removal of grids and text, automatic removal of impurities, effective support for complex microbial statistics, in compliance with the parameter automatic conversion characteristics of GB4789.2-2016.
3. ★Measurement of inhibition zone and analysis of antibiotic potency
1) One click fully automatic simultaneous measurement of all inhibition zones in 6 90mm diameter petri dishes, capable of fully automatic measurement of locally adhered inhibition zones, and automatically obtaining results such as inhibition zone area and diameter in the presence of local text interference. Measurement and analysis take 1-4 seconds, and can save or view various types of analysis data uniformly
2) The petri dish can be placed in any direction. It has the functions of automatic selection of antibacterial zone samples, accurate visualization comparison, and editing. The reading resolution of the antibacterial zone measurement system is ≤ 0.001mm, the highest optical measurement resolution is ≤ 0.0053mm, and the repeated measurement error is ≤± 0.01mm or ≤± 0.1%; The measurement error of potency is ≤± 0.1%; Repeated measurement error of potency ≤ ± 0.1%; Measurement difference between stations ≤ 0.2%
3) Compliant with the Chinese Pharmacopoeia, USP United States Pharmacopeia, and EP European Pharmacopoeia, capable of completing the latestNational Pharmacopoeia (2020 edition)）1、 Second and third doses and combined calculation, as well as analysis of antibiotic potency for all strains. Combination optimization analysis characteristics with potency.
4. Export Query and Upgrade: The analysis of images and data results can be saved and reported directly without manual calculation. The results can be exported directly to Excel or PDF document reports. Equipped with online upgrade feature
5. ★Data traceability and permission protectionSystem administrators can assign usage permissions to more than 10 operators, and operation records can be traced back,Compliant with GLP and GMP requirements.
6. Measurement function: It can also measure the diameter of the antibacterial zone (with a measurement resolution of 0.02mm), and can be automatically or manually calibrated. Can be dragged with the mouse to accurately measure length, angle, radian, area, arc, and any curve
2、 Supply List:
1) 1 set of Wanshen HiCC series automatic colony counting and antibacterial zone measurement and analysis software, lock and accessories
2) 1 autofocus large depth of field 8-megapixel color shooting host (with switchable floating dark field/backlight)
3) Epson Perfection V330 Photo Ultra fine Stereoscopic Color Scanning Imager 1 set
4) HiCC series automatic antibacterial zone measuring instrument dedicated standard plate 1 piece (with 1 authoritative calibration certificate attached)
5) 1 branded computer (Core i5 CPU/8GB memory/256G solid-state drive/19.5 "color display/wireless network card)
Note: This technical proposal includes★The payment must be responsive, otherwise it is a significant deviation